Joghataee, Sadri (2018) Evaluating the effectiveness of auraptene on beta amyloid-induced spatial memory impairment in rats. Masters thesis, Sabzevar University of Medical Sciences.
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Abstract
Introduction: Alzheimer's disease is an advanced and multifactorial neurodegenerative disease, which makes the largest group of learning and memory disorders. This disease is associated with the deposition of amyloid beta (Aβ) in the extracellular space and the formation of neurofibrillary tangles (NFTs) within the cellular body of neurons. Auraptene is a simple coumarin that performs many protective acts as a neurotrophic and acetyl cholinesterase inhibitor compound in the brain. This study was conducted to evaluate the protective effects of auraptene on the memory improvement in Aβ42-induced model of Alzheimer. Materials and Methods: In this study, 48 male Wistar rats (200-250 g) were used. Animals were divided into 6 groups, 8 animals each; Sham (non-injection cannulation), Negative control (Alzheimer induced by injection of 30 ng Aβ42 to the hippocampus), auraptene solvent as well as three therapeutic groups receiving 5, 10 and 25 mg / kg auraptene for 12 days after Aβ injection. The evaluation of spatial memory performance after Alzheimer's induction was performed by morris water maze behavioral test. Bcl-2 Bax ratio was measured by ELISA method to determine the expression of proteins involved in apoptosis. Results: Intraperitoneal administration of auraptene (5, 10 and 25 mg / kg) in therapeutic groups significantly decreased the time to reach the platform and increased the duration of the presence and distance traveled in the target quadrant compared to Alzheimer induced group. Also, ELISA results showed that expression of Bax protein in treatment groups was significantly lower than in Negative control group. While a significant increase was observed in the expression of Bcl-2 protein. Also, the ratio of Bax / Bcl-2 showed a significant decrease in the hippocampus of Auraptene treated groups. Conclusion: This study showed the memory enhancement effect of auraptene after induction of Alzheimer's disease. It seems that this effect could be through the increased expression of Bcl-2 protein and decrease in the expression of Bax protein.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Alzheimer's, Amyloid beta, Auraptene, Spatial memory |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Medicine |
| Depositing User: | Saeed Shoja |
| Date Deposited: | 24 Dec 2018 06:45 |
| Last Modified: | 20 Jan 2019 04:45 |
| URI: | http://eprints.medsab.ac.ir/id/eprint/766 |
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