Akbari Zahmati, Amir Hossein and Khori, Vahid and rezaei shahmirzadi, Arash and Abolhasani, Mohammad Mehdi (2017) chemical methods in decellularization process and its effects on extracellular matrix (ECM): narrative review. Journal of Biomedicine and Health, 2 (2). pp. 28-36.
Full text not available from this repository.Abstract
Objective :Extracellular matrix (ECM)produced by tissue decellularization processes As a biological scaffold Due to its unique properties compared to other scaffolds for migration and implantation of stem cells have been used successfully in the field of tissue engineering and regenerative medicine at the last years. Methods: for this review we searched in google scholar, medline, scopus, sciencedirect .we used extracellular matrix(ECM),biologic scaffold ,decellularization ,chemical methods, tissue engineering as keywords. We selected articles have been published from almost 2000 to 2016Results: 15 full texts and 97 abstract was reviewed. To obtain a intact ECM with preservation of ECM bioactivity. Employing an optimization method to minimize damage to the ECM Ultrastructure As a result of the lack of reduction in mechanical properties and also preservation of important proteins such as laminin, fibronectin, GAGs, growth factor is required. Various methods, including methods of chemical, physical and enzymatic studied and tested. But on each of these methods can have undesirable effects on ECM .Theobjective of this manuscript is to provide an overview of the chemical decellularization methods , Evaluation of decellularizad ECM and the potential affect of the chemical decellularization agents on the biochemical composition, ultrastructure, and mechanical behavior of the ECM scaffold materials.production an intact ECM with preservation of biochemical composition and mechanical behavior requires a correct choice regardingagents and techniques used in decellularization process.Conclusion: It is suggested that insted of the SDS which have high strength degradation we can use zwitterionic separately or in combination with SDS.TBP due to its unique properties can be used in decellularization process.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Saeed Shoja |
| Date Deposited: | 18 Feb 2018 09:34 |
| Last Modified: | 18 Feb 2018 09:34 |
| URI: | http://eprints.medsab.ac.ir/id/eprint/50 |
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