Tahmasebi, Hamed and Bokaeian, Mohammad (2018) Molecular detection of 16s rRNA, qacA / B, smr in Clinical Isolates of staphylococcus aureus methicillin resistance. Journal of Sabzevar University of Medical Sciences, 24 (5). pp. 367-374.
Full text not available from this repository.Abstract
Objectives: Disinfectants and antiseptics agents of the past were used for disinfect equipment and surfaces. In some cases, these substances ineffective by a series of specific efflux proteins that are present in bacteria. QqcA / B and smr such as most important genes those can be expression more efflux protein in strain of methicillin-resistant staphylococcus aureus (MRSA). The aim of this study was to investigate the genes qacA / B and smr is MRSA strains.Methods: 89 isolates from various clinical samples were early identified by biochemical test. Initial screening was performed by Cefoxitin disk diffusion (30 µg) and then detection of mecA, smr and qacA/B gens was done by polymerase chain reaction (PCR). Finally, were analyzed results by using chi-square tests and t-test.Results: Out of 89 staphylococcus aureus isolates, 59 isolates were identified methicillin-resistant by initial phenotype test. Among those, 50 isolates (17/56) have respectively mecA gene, 14 isolates (73/15) smr gene and 29 isolates (58/32) qacA / B gene. Using by statistical tests and p.value test, was obtained the relationship between qacA / B and mecA genes.Conclusions: According to the results of the studie of prevalence of genes in Zahedan and high frequency of MRSA strains, should pay more attention to the use of disinfectants agents.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Saeed Shoja |
| Date Deposited: | 18 Feb 2018 08:55 |
| Last Modified: | 18 Feb 2018 09:09 |
| URI: | http://eprints.medsab.ac.ir/id/eprint/37 |
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