The role of serotonin and its receptors on anti-convulsant effect of curcumin in seizure induced by pentylenetetrazol in male mice.

Arbabi Jahan, Ahmad (2017) The role of serotonin and its receptors on anti-convulsant effect of curcumin in seizure induced by pentylenetetrazol in male mice. Masters thesis, Sabzevar University of Medical Sciences.

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Abstract

Abstract: According to the World Health Organization, nearly fifty million people are diagnosed with epilepsy. About 30 percent of patients do not respond to current drug treatment. Curcumin is an extract of turmeric, demonstrated therapeutic properties effective for seizure control, although the exact mechanism for it, is not mentioned. Objective: In this study, the serotonin pathway and its receptors in the anti-convulsant mechanisms of curcumin in mice was evaluated. Methods: 110 mice were randomly divided into 11 groups (n = 8) groups. The first group received PTZ (80 mg/kg) with curcumin solvent and PCPA solvent. second group received PCPA solvent then 30 minutes after, it was given curcumin (150 mg/kg ) and 25 minutes after curcumin, PTZ injected, third group received PTZ, PCPA (100 mg/kg) and curcumin solvent, fourth group Received PCPA, PTZ, and curcumin. Fifth group animals, first, received NAD-299 (1 mg/kg), RS-102221 (5 mg/kg), SDZ205-557 Hydrochloride (1 mg/kg), and SB 269970 (10 mg/kg) after injection of the antagonists, curcumin was injected intraperitoneally and 25 minutes later of curcumin injection, PTZ was also injected and Seizure activity was investigated for 25 minutes. Sixth group, animals received NAD-299, curcumin and PTZ. The animals of groups seventh, eighth and ninth also, respectively received 5HT2C, 5HT4 and 5HT7 with curcumin and PTZ. The tenth group received PTZ with 5-HT2C antagonist, 5 minutes after 5-HT2C antagonist, PTZ was injected and The Eleven group received PTZ with 5-HT4 antagonist, 5 minutes after 5-HT4 antagonist, PTZ was injected. Results: Curcumin significantly reduces the PTZ-induced seizure severity (except in quantities Death and falling) and PCPA significantly increased the PTZ-induced seizure severity. Depletion of serotonin by PCPA, not only inhibits anticonvulsant function of curcumin (except for the components of tonic seizures and to a lesser extent during tonic-clonic seizure) but also reversed some of anti-convulsant effect of curcumin. 5-HT1A Antagonist, inhibited anticonvulsant action of curcumin effects. 5-HT2C Antagonist inhibited anti-convulsant effect of curcumin in quantity during tonic-clonic seizure. 5-HT4 Antagonists, reduced power anticonvulsant effect of curcumin in quantity duration of tonic seizures. 5-HT7 antagonist strengthened anti-convulsant effect of curcumin in some quantitie and reduce the seizure severity compared to other antagonists as well as PTZ group. In the gene expression analyzes with REAL-TIME PCR, curcumin reduced the expression of 5-HT7. Conclusion: Curcumin by increasing the serotonin levels in the brain, causing imposition of anti-convulsive actions through receptors 5-HT1A, 5-HT2C and 5-HT4 is. It also strengthens their effect by reducing the expression of 5-HT7. Key Words: Serotonin Receptors, Seizure, Curcumin, Pentylenetetrazole.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Serotonin Receptors, Seizure, Curcumin, Pentylenetetrazole.
Subjects: Q Science > QP Physiology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: Saeed Shoja
Date Deposited: 29 Apr 2018 09:11
Last Modified: 09 Jan 2019 07:15
URI: http://eprints.medsab.ac.ir/id/eprint/345

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