Rajabzadeh, Nadia and Naeemipour, Mohsen and Seyedabadi, Mohsen (2017) Multiplex PCR assay for the simultaneous detection of bacterial pathogens in rainbow trout. Aquaculture International, 25 (4). pp. 1569-1575. ISSN 0967-6120
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Abstract
Accurate, rapid, and specific methods are required to identify pathogens for controlling bacterial diseases in fish. Various microbiological and biochemical methods are used to isolate and identify pathogens, but they are not adequately efficient and/or accurate. For example, distinguishing between Streptococcus iniae and Lactococcus garvieae is difficult via microbiological tests. Moreover, these bacteria often cause concurrent infections. Therefore, early and accurate identification of bacterial pathogens in trout is of utmost importance to prevent possible damage. Because of this, the multiplex polymerase chain reaction (multiplex PCR) method was optimized for simultaneous identification of bacterial infections caused by Yersinia ruckeri, S. iniae, and L. garvieae, three harmful bacteria of great importance in aquaculture. Multiplex PCR reaction was optimized on control samples, and the sensitivity and specificity of the test were evaluated. Multiplex PCR reaction was then performed on DNA isolated from samples collected from fish-breeding farms and transported to the laboratory. Multiplex PCR was found to be an accurate and sensitive method to identify simultaneously different species of bacteria.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Saeed Shoja |
| Date Deposited: | 26 Feb 2018 07:07 |
| Last Modified: | 26 Feb 2018 07:07 |
| URI: | http://eprints.medsab.ac.ir/id/eprint/206 |
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